【文獻(xiàn)標(biāo)題】Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto’s thyroiditis ａnd Systemic lupus erythematosus

【作者】BING TAN，WEIXIN YUAN，JINYING LI，et.al

【作者單位】浙江大學(xué)（Zhejiang University）

【文獻(xiàn)中引用產(chǎn)品】

小鼠白介素17A（IL-17A）elisa試劑盒

小鼠γ干擾素（IFN-γ）ELISA試劑盒

小鼠白介素4（IL-4）ELISA試劑盒

小鼠白介素10（IL-10）ELISA試劑盒

小鼠轉(zhuǎn)化生長(zhǎng)因子β（TGF-β） ELISA試劑盒

【關(guān)鍵詞】cell therapy, experimental autoimmune thyroiditis, human amniotic epithelial cells, Hashimoto’s thyroiditis，immunomodulatory, Systemic lupus erythematosus

【DOI】https://doi.org/10.1016/j.jcyt.2018.04.001

【影響因子(IF)】3.84

【出版期刊】《International Society》

【產(chǎn)品原文引用】

Serological assays

Sera cytokine levels of IL-17A, IFN-γ, IL-4, IL-10 and TGF-β of EAT ａnd SLE mice were measured by ELISA. All ELISA kits were from Hengyuan Biological Technologies (Shanghai, China). Specific experimental steps were performed according to the manufacturer’s instructions.

Cytokine production in vitro

Splenocytes of EAT mice at the peak of disease (35 day) were stimulated with pTg in the presence or absence of hAECs. Cell-free culture supernatants were collected after 48 h by centrifugation. Cytokine levels of IL-17A, IFN-γ,IL-4, IL-10 and TGF-β were measured by ELISA(Hengyuan BiologicalTechnologies, Shanghai, China).

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原創(chuàng)作者：上海恒遠(yuǎn)生物技術(shù)發(fā)展有限公司