【文獻標題】GR/HDAC2/TGFβR1 pathway contributes to prenatal caffeine inducedosteoarthritis susceptibility in male adult offspring rats

【作者】Jing Li，Hao Xiao，Hanwen Luo，et.al

【作者單位】武漢大學基礎醫(yī)學院（Wuhan University School of Basic Medical Sciences）

【文獻中引用產(chǎn)品】

試劑-藏紅O

試劑-固綠FCF

【關(guān)鍵詞】Caffeine，Osteoarthritis，Glucocorticoid，Transforming growth factor β receptor 1，Histone modification

【DOI】https://doi.org/10.1016/j.fct.2020.111279

【影響因子(IF)】3.78

【出版期刊】《Food ａnd Chemical Toxicology》

【產(chǎn)品原文引用】

Materials

Caffeine (CAS #58-08-2, > 99% purity) was purchased from SigmaAldrich Co., Ltd. (St Louis, MO, USA). Safranin-O (CAS. NO. 477-73-6)and fast green (CAS. NO. 2353-45-9) were obtained from Hengyuan Biotech Co., Ltd. (Shanghai, China). RIPA lysis buffer ａnd BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China). An enzyme-linked immunosorbent assay (ELISA) kit for detecting serum corticosterone concentration was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Reverse transcription and

real-time quantitative polymerase chain reaction (RT-qPCR) kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). The oligonucleotide primers for rat RT-qPCR genes (PAGE purification)were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).Chondroitin sulfate sodium salt from shark cartilage, DMEM/F12 (1:1)and fetal bovine serum (FBS) were supplied by Gibco (St Louis, MO,USA). Polyclonal rat antibodies including TGFβR1 (ab31013), SMAD2/3 (ab232326), SOX9 (ab185230), HDAC2 (ab213700), aggrecan (ab36861) ａnd Col2 (ab34712) were purchased from Abcam plc.(Cambridge, Cambridge shire, UK). The histone deacetylase 2 (HDAC2)siRNA oligo was purchased from Gene Pharma Co. (Jiangsu, China).Other chemicals ａnd agents were of analytical grade.

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